Combusken's "Bulk Up" attack says "during your next turn, each of Combusken's attacks does 30 more damage to the Defending Pokemon". Does that mean the effect of "Bulk Up" only works if both Combusken AND the Defending Pokemon remain active?

The effect of Bulk Up only resides on Combusken's attacks. If the Defending Pokemon retreats, evolves, etc. the effect still remains on Combusken.

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